[DIYbio] Re: DIY chemostat

Speaking of a OD600 meters... I saw this little gadget demonstrated at a conference not too long ago:

http://buglab.com/docs/BugLab_ODScannerSpecSheet.pdf

Essentially, it's a handheld scanner that you can just push up against the side of a shake flask to measure the OD. You only need 3cm of liquid too. Looks like they're using light reflected from an array of IR laser diodes, and a response that is very linear with cell density.

On Wednesday, November 14, 2012 11:04:46 PM UTC-8, AdvancedMudPieMaker wrote:

Poli, thanks for those links. I followed the threads and they were excellent.  The whole community would benefit from breaking this problem down into some of its parts.

1.  A more detailed how-to on building an OD600 meter out of IR LED, a photodetector, and an Arduino board.

2.  A how- to on even a species-specific capacitive spectroscopy meter hosted on an Arduino for live cell monitoring.

3.  A dissolved Oxygen sensor with Circuit. (commercially available for under $200)

4. A means for temperature controlling 200 liter chemostat--heating and cooling

5. A method for sterilizing large volumes of growth media.

Thanks to all who posted inside of Poli's links.  I have lab space and most bio equipment but fall short on the above.  Stil scanning ebay and other used equipment sources.

Best to all,

Alan

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