Re: [DIYbio] Re: Plant Engineering

Brewer's pectinase should work, but I imagine it's less pure than the
lab-grade stuff (if someone has access to both, a comparison would be
awesome!)

I've been thinking for a while now about bugging someone with more
protein-fu than I to help codify a method of separating cellulase from
those hippy digestive aid tablets you find in good hippy healthfood
stores; they often come with pectinase, cellulase, etc. etc., but also
lipase and a bunch of other potentially lethal *ases which render them
useless for plant protoplast engineering, most likely. A set of steps
you could follow to precipitate out unwanted proteins or otherwise
purify the cellulase/pectinase from these tablets/powders would be great
for DIY plant engineers!

Anyways, as far as viability is concerned trust the packaging. If you
need to test if your pectinase is working, you could always add some to
cloudy apple juice and see if it clarifies in a few hours or days! :)

Also FYI: in English Apple/Pear ferments are usually called "cider".

On 01/11/12 14:31, Mega wrote:
>
>
>
> I'm gonna try to make protoplasts within the next week. I've got cellulase
> from my university, but there seems to be no pectinase left.
>
> Can anyone guesstimate if this pectinase will work?
> http://www.ebay.at/itm/Pektinase-Enzym-Maischeverflussiger-50gr-/150737056744?pt=Spezial_Landwirtschaft&hash=item2318a0f3e8
>
> It's for when you want to brew "beer" from apples and pears (we call that
> "fruit beer" Most in German).
>
>
> And, especially: for how long the pectinase powder will work? Can I keep it
> 1 year without decaying? Because there's a lot of it in this package
> (that's fine because of shipment, but i it decays too quickly, it's
> wastement)
>
> thanks ;)
>

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