Re: [DIYbio] Re: Plant Engineering

It'll probably release some at least, or it'll weaken them for some
other process.. for full protoplasts though, you'll probably need pectinase.

Tip: protoplasts are apparently pretty fragile, so centrifugation could
potentially kill them. I've read about an old-school technique for
avoiding this, where you pipette a layer of sucrose-solution at the end
of the tube before layering the liquid to be centrifuged on top; this
creates a "density cushion" for your cells as they "land" on the surface
of this dense sucrose layer, and may lead to less cell rupture.

Could be bunkum nonsense, and could do nothing at all. Could even make
matters worse! But, there you go. :)

On 01/11/12 21:42, Andreas Sturm wrote:
> Ah thanks.
> Looked up cider, the german article in english say that cider is not
> wide-spread in Austria, we mainly have "Most" (which is closely related).
> http://de.wikipedia.org/wiki/Most_%28Getr%C3%A4nk%29 for Most, there seems
> to be no adequate english homology, as there's not even a translation.
>
> Doesn't relly matter, though, I don't drink that kind of stuff, neither
> beer, cider nor wine ;)
>
>
> When applying just the cellulase and then vortex, will this set free at
> least some cells? I'm gonna try that out anyway ;)
>
>
>
>
>
>
>
>
>
>
>
>
>
> On Thu, Nov 1, 2012 at 10:33 PM, Cathal Garvey <cathalgarvey@gmail.com>wrote:
>
>> Brewer's pectinase should work, but I imagine it's less pure than the
>> lab-grade stuff (if someone has access to both, a comparison would be
>> awesome!)
>>
>> I've been thinking for a while now about bugging someone with more
>> protein-fu than I to help codify a method of separating cellulase from
>> those hippy digestive aid tablets you find in good hippy healthfood
>> stores; they often come with pectinase, cellulase, etc. etc., but also
>> lipase and a bunch of other potentially lethal *ases which render them
>> useless for plant protoplast engineering, most likely. A set of steps
>> you could follow to precipitate out unwanted proteins or otherwise
>> purify the cellulase/pectinase from these tablets/powders would be great
>> for DIY plant engineers!
>>
>> Anyways, as far as viability is concerned trust the packaging. If you
>> need to test if your pectinase is working, you could always add some to
>> cloudy apple juice and see if it clarifies in a few hours or days! :)
>>
>> Also FYI: in English Apple/Pear ferments are usually called "cider".
>>
>> On 01/11/12 14:31, Mega wrote:
>>>
>>>
>>>
>>> I'm gonna try to make protoplasts within the next week. I've got
>> cellulase
>>> from my university, but there seems to be no pectinase left.
>>>
>>> Can anyone guesstimate if this pectinase will work?
>>>
>> http://www.ebay.at/itm/Pektinase-Enzym-Maischeverflussiger-50gr-/150737056744?pt=Spezial_Landwirtschaft&hash=item2318a0f3e8
>>>
>>> It's for when you want to brew "beer" from apples and pears (we call that
>>> "fruit beer" Most in German).
>>>
>>>
>>> And, especially: for how long the pectinase powder will work? Can I keep
>> it
>>> 1 year without decaying? Because there's a lot of it in this package
>>> (that's fine because of shipment, but i it decays too quickly, it's
>>> wastement)
>>>
>>> thanks ;)
>>>
>>
>> --
>> www.indiebiotech.com
>> twitter.com/onetruecathal
>> joindiaspora.com/u/cathalgarvey
>> PGP Public Key: http://bit.ly/CathalGKey
>>
>> --
>> -- You received this message because you are subscribed to the Google
>> Groups DIYbio group. To post to this group, send email to
>> diybio@googlegroups.com. To unsubscribe from this group, send email to
>> diybio+unsubscribe@googlegroups.com. For more options, visit this group
>> at https://groups.google.com/d/forum/diybio?hl=en
>> Learn more at www.diybio.org
>> ---
>> You received this message because you are subscribed to the Google Groups
>> "DIYbio" group.
>> To post to this group, send email to diybio@googlegroups.com.
>> To unsubscribe from this group, send email to
>> diybio+unsubscribe@googlegroups.com.
>> Visit this group at http://groups.google.com/group/diybio?hl=en.
>> For more options, visit https://groups.google.com/groups/opt_out.
>>
>>
>>
>

--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.