I called these folks last week looking for filters, they're pretty much the industry standard for this sort of thing in biotech, they were very helpful to me so maybe you can give a call and ask for higher volume items
On Mon, Dec 31, 2012 at 6:36 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
the pore size is what's going to determine the pressure requirement for forcing the fluid past the membrane, so you could just parallelize with the smaller filters if you can't find a larger diameter one.--On Mon, Dec 31, 2012 at 8:43 AM, Eugen Leitl <eugen@leitl.org> wrote:
Happy $holidays!
A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.
This is typically done with syringe filters,
http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A
but that's on a too small scale, and for rather nonviscous
solutions.
I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/
Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).
At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.
Thanks and a nice 2013 to ye all.
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-Nathan
-Nathan
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