Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On Mon, Dec 31, 2012 at 11:08:14AM -0600, John Griessen wrote:

> and flushing out the assembly
>> for an hour with reverse osmosis water, possibly prefiltered.
>
> How would you be sure that RO water stream is sterile?  Run through tubing
> past a UV light source?

The reverse osmosis plant arguably does UV-sterilization but there's
no accounting for biofilm colonization downstream. I will check using
a microscope, of course, but for time being I consider the reverse
osmosis water to be bacterially contaminated. I will make sure that
everything downstream of the filter will be sterile, and the filter
assembly itself will be sterilized by cold wet sterilization (abovementioned
soaking) prior to flushing it.

In case of success all the biocontaminants will be contained within
the ultrafilter. Residual ones will be killed during autoclaving.

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