Re: [DIYbio] First full DNA extraction, PCR, and gel

Yup heat transfer is the key, but you can avoid mineral oil with glass capillaries since you flame seal them into a closed environment.  Getting the samples out of the capillaries requires some weird aspirator needle thing though, supposedly.  



It does go to show though, that with the glass capillaries you can do a full 30-35 cycle runs in 10-30 minutes, but they were only amplifying a 250bp target.  


That's the chart that shows plastic vs glass, and below you can see how fast the program times are, and as you say, much higher lag with plastic in red.  Pretty nifty stuff.

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