Jordan:
we did the point mutation on eGFP optimized for bacterial expression with the induced vs. non-induced controls and coelenterazine as the positive control with purified Gaussia luciferase from a mammalian expression system. did not work as expected in our hands.
But, are you *sure* that with wild-type GFP there would also not be luciferin production?
It's worth too much to not trying it.... It would be the first (and only) eukaryotic light generating pathway, consisting of only two (!) genes... Too good to be true???
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