Re: [DIYbio] Re: the diy equipment list?

On Tue, Dec 04, 2012 at 09:24:00AM -0600, John Griessen wrote:
> On 12/04/2012 02:33 AM, Josh Perfetto wrote:
>> how long will the liquid nitrogen last?
>
> Longer inside the -20 freezer.

I doubt it would make a large difference, but if
anyone has the time to do the experiment, I would
be interested in the results.

Do not used a locked door on the fridge, but one which will
vent by popping the lid.

> Might make it too cold though.

No, because a dewar is rather good at what it does.

> Would too cold be a problem?
>
> What about frozen CO2? Any cell users data at the temp dry ice
> sublimates (-78.5 °C)?

You would have to store the dry ice in an insulated
box (e.g. styrofoam) to prevent air circulation.
There will be pressure buildup, so it's more suitable
for chest-type freezers which will close by gravity.

Do not stick your head inside your freezer with dry
ice in it, and inhale.

This goes doubly for liquid nitrogen -- you will pass
out suddenly, and fall in, and suffocate.

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