[DIYbio] hot air thermocycler, was Re: OpenPCR

On Thu, Jan 31, 2013 at 1:19 PM, fiaraz <fiaraziqbal@googlemail.com> wrote:
> I was looking into it and spoke to the madlab group in Manchester who
> informed me that it was ok but not as good as the lamp based PCR machine.
> which i am now looking to construct but time is such a rare commodity.

I just took apart an old hot air thermocycler I picked up used for
$40... it has a cheap 500 or 1000W halogen light bulb under a sheet of
aluminum which has been impressed with the PCR tube shape, a cut-out
in the reflector leads to a small squirrel cage fan. The light and fan
are controlled by two solid state relays.

I bet you could replicate something like this using a chinese $8
wal-mart/home-depot flood light, and spray-painted aluminum foil (or
just paint the PCR tube black before cycling)

Here are some pics:
http://nathanmccorkle.com/takeAparts/hybaid/gallery.html

--
-Nathan

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