Thanks! I am replacing the calcium chloride method I have been using with this one, and I am trying it out tonight! I since my altogether project is to create an open plasmid for halobacterium, I will make this the method-of-choice when I get to making competent halobacterium. To me, halobacterium NCR-1 should be as easy as e coli to use!
-Koeng
On Monday, January 28, 2013 2:29:41 AM UTC-8, Cathal Garvey wrote:
Hey Koeng,--
If you're using the PEG/Mg/DMSO method of E.coli transformation, you can
do without DMSO at the cost of an order of magnitude or so in
transformation efficiency.
I've got the protocol without DMSO up here on github:
https://github.com/cathalgarvey/biohacking- protocols
Works quite well using Miralax and generic-brand pharmacy-grade
magnesium sulphate.
On 27/01/13 23:11, Koeng wrote:
> X-gal and competent cell preparation
>
> On Sunday, January 27, 2013 12:53:51 PM UTC-8, Sebastian wrote:
>
> Any particular project in mind?
>
> Sebastian S Cocioba
> CEO & Founder
> New York Botanics, LLC
>
> Sent via Mobile E-Mail
>
> On Jan 27, 2013, at 3:47 PM, Koeng <koen...@gmail.com <javascript:>>
> wrote:
>
>> Thanks guys! Finally I can get some...
>>
>> On Sunday, January 27, 2013 11:43:10 AM UTC-8, Koeng wrote:
>>
>> where do I get this stuff?
>>
>> -Koeng
>>
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