Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

Proteases, etc.

Bacteria already make high-quality cellulose, at a higher purity than
plants do, in fact. It has a slimy consistency but it is exceptionally
strong.

The species most often credited with making "nature's best cellulose" is
Gluconacetobacter xylinus, shortened to G.xylinus, often called
"A.xylinus" in the literature for historical reasons (used to be just
"Acetobacter"). You can get the best strains for cellulose production by
buying a kombucha SCOBY on ebay and isolating for acid-producing
colonies on agar.

There have been some experiments getting E.coli to express the cellulose
operon from G.xylinus, and I seem to recall it worked out OK. So there's
definitely scope for producing it in another host, although G.xylinus is
already very easy to grow and efficient at producing cellulose, so
there's not that much need.

On 28/01/13 13:11, shreyans chordia wrote:
> the idea sounds cool.. it would be really cheap if we could make
> bacteria produce cellulose fibres and then use them to run samples..
> Although a drawback of that would be that the secreted proteins in the
> cellulose fibres will contaminate the sample we want to run.. Anyway to
> overcome that?
>
> On Sunday, 20 January 2013 01:11:43 UTC+1, Nathan McCorkle wrote:
>
> So my thinking is everyone can get paper of some form, but not
> agar/agarose... seems it's not ideal for DNA but it is for amino acids
> and small proteins... it might be useful for DNA or large proteins in
> certain assays though... i.e. yes/no assays or something
>
> here are some things i dug up:
>
> mentions a few paragraphs of history
> https://www.idtdna.com/pages/docs/educational-resources/gel-electrophoresis.pdf
> <https://www.idtdna.com/pages/docs/educational-resources/gel-electrophoresis.pdf>
>
>
> including
> "
> While paper and other solid support materials proved to be an
> advantage over free
> solutions for the electrophoretic analysis of biomolecules, gels were
> adopted later
> because gels not only minimized diffusion better than paper supports
> they actually
> participated in the separation process by interacting with the
> migrating particles.
> "
>
> and referencing this article about Bothrops (pit viper snake) venom,
> but it's in German (I think) and I can't read it (google translate
> doesn't do much either because copy-paste don't keep the sepcial
> characters)
> http://nathanmccorkle.com/pdf/this%20might%20be%20about%20paper%20electrophoresis.pdf
> <http://nathanmccorkle.com/pdf/this%20might%20be%20about%20paper%20electrophoresis.pdf>
>
>
> I got thinking because I saw this chromatography paper for $0.65 per
> foot and mentions "widely used for electrophoresis"
> http://store01.prostores.com/servlet/thescienceshop/the-2012/CHROMATOGRAPHY-PAPER-2cm-Wide/Detail
> <http://store01.prostores.com/servlet/thescienceshop/the-2012/CHROMATOGRAPHY-PAPER-2cm-Wide/Detail>
>
>
>
> thesis on
> "Development of Paper electrophoresis technique for observation of
> microgram quantities of protein" and "electrophoretic and
> ultracentrifugal studies of soluble antigen-antibody complexes as a
> method of determining antibody and antigen valences"
> James T. Bradbury, California Institute of Technology, 1956
> http://thesis.library.caltech.edu/995/1/Bradbury_jt_1956.pdf
> <http://thesis.library.caltech.edu/995/1/Bradbury_jt_1956.pdf>
>
>
> --
> -Nathan
>
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