Interesting.. thats like running ampholytes before loading samples on a pH gradient gel.. only disadv is buffer wastage..
using proteases is not cost effective, also the proteases cannot get to the proteins because of the crystalline packing of cellulose..
how bout exploting the fact that cellulose can take high temps.. then we could ppt proteins out o it maybe.. but it could also clog the pores in the fiber..
gonna lookup bac cellulose processing, although i have a feeling most o it must be under wraps due to patenting :(
On Monday, 28 January 2013 17:45:35 UTC+1, Nathan McCorkle wrote:
-- On Monday, 28 January 2013 17:45:35 UTC+1, Nathan McCorkle wrote:
On Jan 28, 2013 5:12 AM, "shreyans chordia" <shreyans...@gmail.com> wrote:
>
> the idea sounds cool.. it would be really cheap if we could make bacteria produce cellulose fibres and then use them to run samples..
> Although a drawback of that would be that the secreted proteins in the cellulose fibres will contaminate the sample we want to run.. Anyway to overcome that?Pre-run the electrophoresis maybe
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