Re: [DIYbio] Re: Tips for the new guy, so he can avoid posting stupid questions.

How did I miss genefoo?!
Sign me up for a 4-pack! :D
(Posting in offline mode, will hit up genefoo ASAP and place an
order..assuming you guys can ship overseas!)

On 28/01/13 05:51, Mac Cowell wrote:
> Hey everybody, Mac here. I run genefoo.com <http://genefoo.com> and sell
> the fabulous $44 pipettes. If you buy a box, you will see the are
> marked as "The Ultimate Pipetting Experience" :) I'm happy at least to
> sell them at the ultimate price. It's up to you to decide if it really
> is the ultimate pipetting experience :)
>
> As Sebastian suggested, I import them from Shanghai. So far, they seem
> great. I am still trying to figure out the design pedigree of them, but
> I think they are based on finnpettes from the 90s.
>
> My goal with genefoo is to lower the barrier to entry for a lot of basic
> molecular bio lab equipment, in an open-source way when possible, and
> these pipettes are genefoo's first product. Rest assured that if they
> don't work for you, I will do everything it takes to make them better,
> and together we'll find the best pipettes for diybiologists. Maybe
> importing them won't even make sense in the long run and used sets from
> ebay will be the best way to acquire them for users on a shoestring
> budget. Time will tell, and it will tell faster with your help.
>
> By the way, I want to offer a 5% discount on a set of 4 pipettes,
> Please email me at sales@genefoo.com <mailto:sales@genefoo.com> to set
> that up.
>
> Cheers
> Mac
>
>
> On Sun, Jan 27, 2013 at 9:26 PM, Sebastian S. Cocioba
> <scocioba@gmail.com <mailto:scocioba@gmail.com>> wrote:
>
> Original equipment manufacturer. Its made in china but via ISO
> standards so I would not worry. Normally if you buy a product
> straight from the source its cheaper but when the source is China
> people tend to think cheap and flimsy. If these really are able to
> match the ISO standards then I am down to buy a whole set...
>
>
> Sebastian S Cocioba
> CEO & Founder
> New York Botanics, LLC
>
> Sent via Mobile E-Mail
>
> On Jan 28, 2013, at 12:23 AM, Avery louie <inactive.e@gmail.com
> <mailto:inactive.e@gmail.com>> wrote:
>
>> OEM?
>>
>> On Mon, Jan 28, 2013 at 12:20 AM, Sebastian S. Cocioba
>> <scocioba@gmail.com <mailto:scocioba@gmail.com>> wrote:
>>
>> Wow thats a fantastic price! Is the catch just that its OEM?
>> Either way as a beginner set thats wonderful. Three pipettors
>> for the price of an okay one on sale. If I had a time machine...:P
>>
>>
>> Sebastian S Cocioba
>> CEO & Founder
>> New York Botanics, LLC
>>
>> Sent via Mobile E-Mail
>>
>> On Jan 28, 2013, at 12:15 AM, Avery louie
>> <inactive.e@gmail.com <mailto:inactive.e@gmail.com>> wrote:
>>
>>> Just a plug for one of our fellow DIYBIO folks, Mac Cowell
>>> sells super affordable pipettors. They should be worth a
>>> shot- they are very competitively priced: super affordable
>>> pipettors <http://genefoo.com/products/pipettes>. Your other
>>> best bet is to get a /set/ of gilsons (kind of the mo-bio
>>> standard) off ebay.
>>>
>>> --Avery
>>>
>>> On Sun, Jan 27, 2013 at 11:49 PM, Koeng <koeng101@gmail.com
>>> <mailto:koeng101@gmail.com>> wrote:
>>>
>>> I was in the same boat as you once, except until recently
>>> I haven't discovered DIYbio so all I had to guide me is
>>> the experiment I wanted to complete and a computer to sit
>>> hours upon hours infront. I don't have a firm
>>> understanding of most complex biology/biochemistry, I
>>> haven't even passed high school! Lab technique is what I
>>> do know :). But I just set a goal on something cool and
>>> began collecting things.
>>>
>>> My recommendation- Just get a goal and start. Start
>>> getting decent equipment as the experiment needs. I got
>>> my plasmids from Carolina, cheap and easy, even though I
>>> only got pGreen and pVIB. When I started transformations
>>> I didn't even have a water bath, or an incubator, or
>>> pipettes. I didn't even have a metal inoculating loop!
>>> But to make transformations easier, I started to get
>>> these things. Then it just started piling up and I got
>>> into viruses, then where I am now, plasmids. Also, get
>>> GOOD, let me repeat this GOOD LB agar. Make your own! Do
>>> anything and avoid buying it from p212121 (They have
>>> great regents, just check out GoldBio first). Mine sucks
>>> and that, strangely, is the biggest thing holding me back
>>> from most my experiments. GET GOOD AGAR PLATES
>>>
>>> Some software to get: ApE and SnapGene. ApE is great for
>>> making DNA because it is almost like a text editor, and
>>> snapgene is just so easy! I highly recommend the free
>>> trial when you start your first major project.
>>>
>>> Mo bio: This is where you can make a living getting
>>> samples. For that reason I always buy from them because I
>>> get to try their products first. This can supply you for
>>> quite a while.
>>>
>>> And finally I will try to keep up about plant
>>> transformation. I want to try it too :)
>>> -Koeng
>>>
>>>
>>>
>>>
>>> On Sunday, January 27, 2013 6:42:38 PM UTC-8, Avery
>>> Ashley wrote:
>>>
>>> Hey guys, I'm relatively new to this. I'm only in my
>>> second year at college (community college at that),
>>> but I have a passion for biology, lots of free time
>>> and a little money free to spend on a good hobby.
>>> I've never worked in a real lab, so there's a lot of
>>> techniques I need to learn and I don't want to be
>>> constantly posting here about them and wasting your
>>> time.
>>> I've gotten a makeshift incubator and centrifuge, and
>>> a legit thermocycler and electrophoresis chamber but
>>> I really don't know how to use any of it very well.
>>> Basically, I have everything I need I just need to
>>> learn how to use it properly.
>>> I want to work mostly on plants, and my goal is to
>>> be able to insert genes in them by the end of summer
>>> (not to accomplish anything really, just be able to
>>> do it).
>>>
>>> Where do i start?
>>> I have a relatively firm understanding of basic
>>> biology and biochemistry, I'm only looking for a
>>> place to learn lab technique.
>>> And I'm pretty much stuck on my computer.
>>> Thanks for the help, you guys are one of the most
>>> understanding and helpful community's I've ever
>>> encountered on the internet, and I'm proud to be a
>>> part of all this.
>>>
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