Hi, don't feel like you are a leach, the whole point of this list is to share info!
In fact, you may have stumbled upon something here. In your next email to them, if all goes well, maybe you could mention the DIY list and that you may be sending more potential customers to them, and maybe they could setup a coupon sort of deal...or have some way of knowing that if you list DIYBio, you can buy kits with enough materials for 1-2 reactions per kit instead of the full 10. Might as well have 1 or 2 extra rxns in case one messes up.
I'd be interested in that blue and green protein transformation to be honest.
It seems like all the kits come with the required things...but maybe they are reducing the price with the forethought that you already have agar to pour gels, or a DNA stain to use (since multiple kits use the stain). I'm a little confused to, they weren't specific on what exactly they'd give you with the reduced price.
A good buffer to have on hand is TBE or TAE, used for pouring and running gels, it's pretty cheap. You can stain gels with methylene blue for white light visualization, or GelGreen or SYBRGreen for LED/UV visualization.
If I'm not mistaken methylene blue stain usually takes a few hours to overnight to visualize the DNA, I've got GelGreen which worked fantastic for UV illumation, and I know others have had success with blue LED illumination but I"ve yet to get a blue LED.
I can't find all the links atm but, my most recent purchases have been from
http://www.phenixresearch.com/products/lab-supplies.asp
for GelGreen, and agarose. You might also find your tubes, tips, EDTA, tris, or premade TBE or TAE on there as well.
Maybe try to get prices for 2-3rnx per kit, and ask if they'd still provide everything they say they will on the kits main page, just in smaller quantities.
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