If you really feel out of your element and want kind of a guiding hand, edvotek sells kits that range from bacterial transformations to gene amplification, ligations, etc. The cost is obviously more than getting the disposables/ingredients yourself but the packaged convenience is worth it IMO. Sometimes, by not knowing what you need, you end up overspending on raw materials. Amazon.com has an edvotek listing in case they wont ship to your address. They mostly deal with schools so the listing is a work-around. The alternative to kits would be much more time consuming since you would have to learn what everything does and how much you need and most importantly why on your own. The internet is a beautiful place. If you have the time you can learn everything you need at the click of a mouse. Benchfly is great. Jove has some public access videos too. Its like a research article but in video format which may be helpful at times. Openwetware is another great resource. And of course just ask the list. If you just try to do a google search on the topic before posting, you might either find your answer or narrow your question significantly. If not then ask away. There are tons of people who enjoy helping others in this community. We all had to start somewhere! :) good luck!
PS: nice buy on the gene amp. I got its grandfather the 9600. What a giant machine! As long as it gets the job done, eh? :)
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
Thanks for the reply, I'll check it out.I bought a used perkin gene amp 2400. I guess I should have said the chamber is makeshift too, its just a little plexiglass box with battery hooks, not even sure if it'll work haha.--
On Sunday, January 27, 2013 9:51:03 PM UTC-5, Dakota wrote:I'll try to find some more things tomorrow, but I found this website really nice, and stumbled on it a year or two ago. What thermal cycler and gel box did you end up going with?It's basically youtube, but ALL lab techniques. Sometimes you can find good stuff on youtube, or MIT open course ware, but some times on youtube it's explained poorly, or it's just text layed over a video and not really informative. Most of the videos I've seen on benchfly can be really detailed and informative, and explain not just how to do something, but why you do certain things (all the ins and outs and nuances)
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