[DIYbio] Re: What DNA separation techniques other than electrophoresis?

I think the main problem with DNA and acquiring single base pair resolution on separation is that DNA has "structure" whether it is coiled or wrapped around itself or anything. This makes most electrophoretic system non workable unless one is denaturing the DNA in some way. For sequencing gels this is often done with Urea or GdmHCL and SDS.

Denaturing HPLC is pretty clever technique that people use to determine SNPs and the like: http://www.ncbi.nlm.nih.gov/pubmed/20938830


On Wednesday, May 11, 2011 2:05:20 PM UTC-5, Nathan McCorkle wrote:

Does anyone know of ways other than electrophoresis of DNA to
separate? The problems with long run times and exhaustion of buffer
seem like they could be alleviated... but how? Maybe something like
flow cytometry, but in a much smaller capillary? Maybe the DNA could
be ligated to something like sialyl-lewis-X, and the capillary coated
in CD62P (protein that binds SLX) to slow down the DNA during its
flow... then detection could proceed with UV spectroscopy (the
capillary would need to be very thin to allow differentiation of
single/few molecules)

What other ideas do people have?

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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