Yeah, we got pGreenII with kanamycin and firefly luciferase. 35S GFP is aslo delivered with it, and must be ligated into. Problem: GFP has EcoRV blunt ends.
And we just got 1ug, so we need to amplify it. Because on the gel there were two bands for pGreenII, one maybe supercoiled, one unopened. Maybe the enzyme is too weak already, and we didn't really use much of it.
However, the Genome Compiler GlowingPlant kickstarter project will be started within a few weeks, and then we get a bioluminescence - not just GFP - design!! Thus, there will hardly be time to use pGreen-GFP-Luc because, obviously, bioluminescence is much much better and it's gonna have highest priority!!!
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