Here is some more info on next generation sequencing. Illumina uses a reversible terminators, and other groups have developed other chemistries. Basically, the 3' OH gets blocked, and then the blocking group is chemically or enzymatically cleaved:
http://jimlund.org/blog/pics/Metzker_2009.pdf
http://jimlund.org/blog/pics/Metzker_2009.pdf
The other next generation sequencing companies (454, Ion Torrent, SOLID) do the sequencing differently, this talk has some useful slides on the other technologies:
https://www.iths.org/sites/www.iths.org/files/eventmedia/ITHS_2ndGenerationSequencingTalk.pdf
https://www.iths.org/sites/www.iths.org/files/eventmedia/ITHS_2ndGenerationSequencingTalk.pdf
The Church lab's papers are worth looking up, they developed a DIY next gen system using emulsion PCR:
http://arep.med.harvard.edu/gmc_pub.html
Esp: Challenges of Sequencing by Synthesis (2009), Overview of DNA sequencing strategies (2008), and Polony DNA sequencing.(2006), and Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome (2005)
http://arep.med.harvard.edu/gmc_pub.html
Esp: Challenges of Sequencing by Synthesis (2009), Overview of DNA sequencing strategies (2008), and Polony DNA sequencing.(2006), and Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome (2005)
Jim Lund
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