On Feb 28, 2013 2:50 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>
> On Thursday, February 28, 2013 1:53:37 PM UTC-8, Nathan McCorkle wrote:
>>
>> Cool! My thinking is throw the reversible terminators in a pipeline
>> with terminal transferase, and voila, green chemistry DNA synthesis
>> (well depending on the reversible terminators!)
>
>
> That is precisely how a lot of DNA synthesis works. It's also how a lot of microarrays get synthesized, by using light-directed synthesis directly on the chip.
I thought that was normal phosphoramidite synthesis with a photogenerated acid for activation
In fact, there are strong links between easily/reliably achievable read lengths in DNA sequencing, oligo lengths in DNA synthesis, and probe lengths in microarrays.
>
> Most of the current NGS methods are also called "sequencing-by-synthesis"
Yeah but they use a template, terminal tranferase doesn't depend on a template to extend ssDNA
precisely for this reason. A counter example would nanopore sequencing, where the DNA is read by pulling it through a pore, rather than by synthesizing a complementary strand one base at a time.
>
> Patrik
>
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