Re: [DIYbio] Re: Geneome Sequencing Costs

Cool! My thinking is throw the reversible terminators in a pipeline
with terminal transferase, and voila, green chemistry DNA synthesis
(well depending on the reversible terminators!)

On Thu, Feb 28, 2013 at 7:04 AM, jlund256 <jlund256@gmail.com> wrote:
> Here is some more info on next generation sequencing. Illumina uses a
> reversible terminators, and other groups have developed other chemistries.
> Basically, the 3' OH gets blocked, and then the blocking group is chemically
> or enzymatically cleaved:
> http://jimlund.org/blog/pics/Metzker_2009.pdf
>
> The other next generation sequencing companies (454, Ion Torrent, SOLID) do
> the sequencing differently, this talk has some useful slides on the other
> technologies:
> https://www.iths.org/sites/www.iths.org/files/eventmedia/ITHS_2ndGenerationSequencingTalk.pdf
>
> The Church lab's papers are worth looking up, they developed a DIY next gen
> system using emulsion PCR:
> http://arep.med.harvard.edu/gmc_pub.html
> Esp: Challenges of Sequencing by Synthesis (2009), Overview of DNA
> sequencing strategies (2008), and Polony DNA sequencing.(2006), and Accurate
> Multiplex Polony Sequencing of an Evolved Bacterial Genome (2005)
>
> Jim Lund
>
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--
-Nathan

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