Re: [DIYbio] Re: What DNA separation techniques other than electrophoresis?

On 02/27/2013 11:35 PM, Nancy Liu wrote:

> Please have a look at www.ehsystems.com

Are you saying some additions to a product like CEM-7000 Integrated CE System
would allow separation as well as detection?
Or are you referring to product MFL-4100 Microfluidic System as a starting point?

The CEM-7000 page says 100 PSI can be provided. Does that mean electrophoresis
buffer/gel can be pumped to any of the capillaries in your carousel at 100 PSI
to make the effective length of a capillary longer and separation of bands larger?
Can you switch that cleanly to the next capillary in the carousel and so on till all done?
Clean switching would mean, no air bubble created, buffer same as that in capillary, no dirt introduced.

If one could make a microfluidic valve that unwanted DNA could flow past with none getting stuck there,
one could then open that valve and close the normal exit, then operate the pump to move that DNA band into
the collection channel of your microfluidic plate? Instead of a Tee in the path and a valve at the Tee,
a capillary end that is switchable to seal against a collection port could serve as the "valve".
Another alternative would be to use capillaries with scored snap-off zones, and use the detectors,
electrophoresis applied voltage, and buffer pumping to get the desired DNA in the zone, then stop
and switch to another capillary to process.

The last idea of a scored snap-off zone in a capillary might be the cleanest, lowest contamination way.

So now that idea is patent-proof, right? Since I said it here? :-)

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