i use Gel Red at a 1:10,000 dilution which means 1 microliter for every 10 milliliters of gel volume. I put it into the gel itself instead of staining after ward. More expensive than EtBr-yes, but in my mind worth it. It also seems to be more sensitive than EtBr, so you could possibly get away with a higher dilution (something to look into).
On Tuesday, March 12, 2013 10:22:20 AM UTC-4, phillyj wrote:
I'm looking into using Gel Red dye instead of EtBr. They have--
different versions. I think the 0.5 mL 10000x in water stock solution
(catalog # 41003) is what I should be getting.
I'm wondering how I would use it. It says use at 1:10000 dilution so
does that mean use 0.1uL per 1mL gel volume? If I have a 50mL gel, do
I add 5uL gel red? That would give me 100 gels. Hmm....for $100. Seems
expensive.
They also have 4L of 3x in water for $200. They say dilute to 1x but
don't say what concentration to use in a gel. What do you guys do?
Thanks
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