[DIYbio] Re: PCR end weight

I am trying to assemble a Halobacteria vector for the model organism Halobacteria NRC-1, and not top secret, I want to give the plasmid out for free once I finish xD

I am thinking about doing a miniprep but I have to do a transformation today or else I fear my experiment will not have results in time for my science fair...

Yes they do need crazy salt concentrations, it gets very annoying because the when I pour the agar, everywhere it touches turns into salt :/

I do not believe they have an extra membrane, considering E coli has a protein layer too I think the PEG will still work, however I need to test CaCl method as well to get the best results.

Thanks

On Wednesday, March 13, 2013 8:48:45 AM UTC-7, Koeng wrote:

Hello all!

I am using the gibson assembly and it requires a certain amount of dna, but I don't know how much dna my PCR reaction will create! Is there any equations, or recommendations on how much I should use if in my reaction I used .2 µM of primer in each reaction?

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