Thanks, I think I have to retry the pcr reaction because I do not have a heated lid, and forgot to put oil on it... almost everything was evaporated....
So this time I put cooking oil on the lid and in the wells, and increase the hold times.
Koeng
On Tuesday, March 12, 2013 8:42:28 PM UTC-7, Koeng wrote:
Hey guys,--
I am doing one of my first own pcrs with my machine and tubes, when the sudden realization that the pcr machine filled .5 (I think) ml when I had .2 ml tubes! Does anyone think that it will still work? Anyway, I am on a strict time table, so I am going to PCR it and check the results on Sunday (my UV light doesn't work for my electrophoresis, so much for being cheap and getting storebought blacklight when i lost my good UV light). So who wants to give a guess if it will work or not?
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