[DIYbio] Re: Primer Purification

Yes, if you can PAGE purify and extract the oligo yourself then it will be perfectly fine to use. Many of our customers here at SIGMA do that, to save the extra up-front costs.

On Friday, March 15, 2013 7:53:03 AM UTC-5, Mega wrote:

Hi everyone,
 
 
I was ordering Primers, and one primer costs around 45€ (around 100 bp). Purification is the major cost with 30€ (instead of standard  37.50€).
 
A professor of mine told me that I could make a PAGE gel myselve, thus one primer synthesis would just be 15€.
 
 
Given we would have all the equipment, would that be feasible? Would it be of high enough quality?
 
 
Best,
Andreas

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