Hello everyone!
Currently I am creating a halobacteria vector, and the (hopefully) final version is mutagenesizing in my PCR machine right now.
I have the origin of replication and everything in it, but the gene for chloramphenicol resistance is kinda problematic. I am mutagenesizing an RBS downstream from the gene, but how about the promoter? I am currently using the one from pBeloBac11, common always on promoter.... But how will it work in Halobacteria? Any guesses? Because if it does need a different promoter that is going to be a pain...
Koeng
I will also update when the results come in!
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