Re: [DIYbio] DNA separation by capillary electrophoresis?

On Sat, Mar 2, 2013 at 11:11 AM, John Griessen <john@industromatic.com> wrote:
> On 03/02/2013 12:54 PM, Nathan McCorkle wrote:
>>
>> A fluids guy was telling me a while back that sharp corners can cause
>> pressure drops which can cause precipitation of stuff in solution, and
>> can eventually lead to clogs in a fluid line.
>
>
> If you had snapped a capillary, it would then be dealt with by
> very small flow rates just to push out its contents, so no whipping around
> an edge with
> bernoulli pressure drops.

I think you might have that effect no matter what flow rate, but yeah
pressure drops will certainly be less. It was just something he
mentioned to me once, to keep in mind and watch out for. He wasn't a
microfluidics expert or anything.

>
> I get that score-snapped
>>
>> ends would be perfect or close to being flat
>
>
> Yes, they could be pushed against a port for putting pressure on them to
> empty them.
>
> A robot hand could move them around, and a robot eye could check how far gel
> or liquid is pushed out,
> then stop, the toss the capillary section in trash, flush and clean that
> rubber sealed air pushing port
> occasionally...

Hmm, why not just have the robot wipe the excess gels from the end of
a pre-fab capillary with a disposable wipe? (Rather than snapping it)


--
-Nathan

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