The key for this working is to ensure that the business portion of the PCR tube (the portion containing the reaction volume and a bit above it) makes excellent contact with the PCR machine block. If it fits snug that's great, if there's an air gap the reaction temperature control will be way off. If you have a gap, then in a pinch you might make this work by putting mineral oil (or cooking oil) between the heat block and tube so as to fill in the gap. And you may need to extend your step hold times compared to a 200 ul tube, as it will still take longer for your reaction volume to reach the block hold temperature.
On Tue, Mar 12, 2013 at 8:42 PM, Koeng <koeng101@gmail.com> wrote:
Hey guys,
I am doing one of my first own pcrs with my machine and tubes, when the sudden realization that the pcr machine filled .5 (I think) ml when I had .2 ml tubes! Does anyone think that it will still work? Anyway, I am on a strict time table, so I am going to PCR it and check the results on Sunday (my UV light doesn't work for my electrophoresis, so much for being cheap and getting storebought blacklight when i lost my good UV light). So who wants to give a guess if it will work or not?
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