Re: [DIYbio] Re: cheaper/better transformation - using ultrasound (sonoporation)

Hold yer horses! I wasn't involved in this experiment - just forwarded the picture. I'll let you know as soon as I know any more details.

Yes, the yield looks low, but I doubt they did much (or any?) optimization of the protocol so far. Not too bad for something that worked on the first try!

Patrik

On Friday, March 1, 2013 3:23:57 AM UTC-8, Avery wrote:

well done!  I am also curious.  That looks like pretty low yield for compared to what i normally see from heat shock, but I would like to know more about the protocol.

--A

On Fri, Mar 1, 2013 at 4:42 AM, Mega <masters...@gmail.com> wrote:

Awesome!! How much ug plasmid  did you use?

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.

To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/iKZNHhtAlLgJ.

For more options, visit https://groups.google.com/groups/opt_out.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/x6ZU_su-ySIJ.
For more options, visit https://groups.google.com/groups/opt_out.