I just searched for the genome sequence of firefly. Didn't find any results.... Has this never been done?
What about this: Why not make a genomic library of firefly in Agrobacterium Ti plasmid pGreenII 0049: http://www.pgreen.ac.uk/JIT/pGreenII/T-0049.gif
That would mean, the firefly Luc gene is always present in each colen, and additionally, when the gene for luciferin is present within the restriction fragment, it will make the transformed plant glow then.
Using plants because they are eukaryotic, it may not work in bacteria due to compartimentization. Or would that work better in bacteria, beause everything stays more or less in the cytosol?
AFAI remember, the 35S Promoter gives *some* expression in bacteria too. But the promoter of the Luciferin gene(s) may not work in bacteria...
Just some thoughts...
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