On Sat, Mar 2, 2013 at 2:31 AM, Mega <masterstorm123@gmail.com> wrote:
I just searched for the genome sequence of firefly. Didn't find any results.... Has this never been done?What about this: Why not make a genomic library of firefly in Agrobacterium Ti plasmid pGreenII 0049: http://www.pgreen.ac.uk/JIT/pGreenII/T-0049.gif
Screening the library for activity is the hard part, it needs automation because there are millions or billions of clones (library from genomic DNA). I did this by hand one summer in E.coli for some small molecule using an Xgal blue reporter strain... it was not fun.
I really should write some color selective blob counting software....
-Nathan --
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