Re: [DIYbio] Re: Engineering Plant Bioluminescence (was Re: experimentation)

> However, maybe, just maybe som leaky expression would be enough...
> 1 Photon needs just one ATP when using firefly luciferase instead
> of 60 ATPs for  bacterial luminescence!

Have you got a citation for that? I was under the impression that
bacterial bioluminescence was in the 90-100% efficiency?

> As long as I get plenty of transformants so that each fragment is
> represented at least once, right?

That's what you need high transformation efficiency to guarantee; to
make sure you have clones of an entire genome, you need huge numbers
of transformants.

On 03/03/2013 09:47 AM, Andreas Sturm wrote:
> Why would I care for transformation effiecncy? As long as I get
> plenty of transformants so that each fragment is represented at
> least once, right?
>
> that you can get insect genes to express in a bacteria, that they
> get the correct posttranslational modification to be active, that
> all the genes for the luciferin biosynthesis pathway are adjacent,
> and close enough together to fit in the bacteria in one piece, and
> that the bacteria has the right metabolic precursors available.
>
>
> Well, it could be that precursors are already present. Sometimes
> living organsms use what is available instead of inventing an
> entirely new metabolic pathway...
>
>
> However, maybe, just maybe som leaky expression would be enough...
> 1 Photon needs just one ATP when using firefly luciferase instead
> of 60 ATPs for  bacterial luminescence!
>
> So if the attached promoter gives just 1/60 of what the pVIB
> promoter gives, it would be brightly visible. If it's less, it
> would still be measurable...
>
>
>
>
>
>
> On Sun, Mar 3, 2013 at 8:38 AM, Nathan McCorkle <nmz787@gmail.com

> <mailto:nmz787@gmail.com>> wrote:
>
> On Sat, Mar 2, 2013 at 9:02 AM, Andreas Sturm

> <masterstorm123@gmail.com <mailto:masterstorm123@gmail.com>>

> wrote:
>> Screening the colonies for activity in this case would be quite
> easy. Turn
>> off the lights, and the ones which don't glow are discarded :D
>>
>
> Unless you want to calculate transformation efficiency, total
> transformants, etc... in that case you need to count ALL bugs, not
> just the interesting ones!!!
>
>>
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