Re: [DIYbio] Re: What DNA separation techniques other than electrophoresis?

I don't know what to say because I don't know what exactly your
requirement. That why I just suggested you a website, maybe it will
create you idea after you go through it. All I want to say people
have answered you already.

On Thu, Feb 28, 2013 at 10:27 AM, John Griessen <john@industromatic.com> wrote:
> On 02/27/2013 11:35 PM, Nancy Liu wrote:
>
>> Please have a look at www.ehsystems.com
>
>
> Are you saying some additions to a product like CEM-7000 Integrated CE
> System
> would allow separation as well as detection?
> Or are you referring to product MFL-4100 Microfluidic System as a starting
> point?
>
> The CEM-7000 page says 100 PSI can be provided. Does that mean
> electrophoresis
> buffer/gel can be pumped to any of the capillaries in your carousel at 100
> PSI
> to make the effective length of a capillary longer and separation of bands
> larger?
> Can you switch that cleanly to the next capillary in the carousel and so on
> till all done?
> Clean switching would mean, no air bubble created, buffer same as that in
> capillary, no dirt introduced.
>
> If one could make a microfluidic valve that unwanted DNA could flow past
> with none getting stuck there,
> one could then open that valve and close the normal exit, then operate the
> pump to move that DNA band into
> the collection channel of your microfluidic plate? Instead of a Tee in the
> path and a valve at the Tee,
> a capillary end that is switchable to seal against a collection port could
> serve as the "valve".
> Another alternative would be to use capillaries with scored snap-off zones,
> and use the detectors,
> electrophoresis applied voltage, and buffer pumping to get the desired DNA
> in the zone, then stop
> and switch to another capillary to process.
>
> The last idea of a scored snap-off zone in a capillary might be the
> cleanest, lowest contamination way.
>
> So now that idea is patent-proof, right? Since I said it here? :-)
>
>
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