Re: [DIYbio] Re: Where to get primers cheaply

Why is your promoter so big... I mean like normally promoters are like 35 bps..

http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119

Are you getting a operator and a RBS too?  Also a recommendation from me is to preform mutagenesis if your strands are that big.

-Koeng
On Sunday, March 31, 2013 12:17:52 PM UTC-7, Mega wrote:

Hi,

No, I meant as a primer for PCR, where promoter is already attached...

On Sun, Mar 31, 2013 at 8:57 PM, SC <stac...@yahoo.com> wrote:

For the 200-mer, I'm assuming you mean a double-stranded fragment you can use for homologous recombination - is that what you had in mind?  I don't think a 200-mer can be used as a PCR primer.

 

You can have such fragments synthesized by Genscript.

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