Re: [DIYbio] Re: Easiest transformation

Hahaha. It is science! It is not a myth. 

Hypoosmotic stress has been a well observed phenomenon for a long time. If you don't believe me grab a microscope and view it. Personal anecdotes are not science. Who knows why the bacteria survived? 



On Thu, Apr 11, 2013 at 8:54 AM, Mega <masterstorm123@gmail.com> wrote:


On Thursday, April 11, 2013 3:40:47 PM UTC+2, Josiah Zayner wrote:
Incubating bacteria in water will kill it due to osmotic stress. they will explode.


No, surely not. I once suspended a colony of E.Coli carrying pVIB plasmid in deionized (!) water (in an Eppi) and plated it on agar. There was a bacterial lawn. I think that is a myth. 

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