Re: [DIYbio] Re: Non-Platinum Electrophoresis Electrodes That Don't Degrade

I have a couple gel boxes made with nichrome wire. They're not
perfect. After about 3 months of daily use they start to look
corroded but considering it's easy to work with and is super cheap
(around 10 dollars for 100 feet of 26 gauge wire) I think it's a good
option to consider.

-cory

On Sun, May 26, 2013 at 10:55 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
> I would not call one dollar per cm "pretty expensive". That's what, $20-40
> per gel box? How does that compare to the power supply you're using to run
> the electrophoresis?
>
> I've used stainless steel wire without much noticeable corrosion during a
> single run. But yeah, I would expect you'll need to replace the wire every
> couple of gels at least. If your gel box is designed such that it only takes
> a few minutes to swap out fresh stainless steel wires for every gel, that
> could defintely be an option.
>
> Anyone see any side effects you might get from having some additional iron
> or chromium ions in the buffer, from corroding stainless steel electrodes?
>
> Patrik
>
>
>
> On Sunday, May 26, 2013 11:29:19 AM UTC-7, Josiah Zayner wrote:
>>
>> I have been testing out a bunch of different metals and they either
>> oxidize to death on the minutes timescale or in the case of stainless steel
>> turn the buffer orange/brown.
>>
>> Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a
>> cheaper metal at 100V or greater with TAE?
>>
>> I have tried:
>> Nickel
>> Stainless Steel 430
>> Tinned Copper
>>
>> Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone
>> ever tried these?
>>
>>
>> Thanks,
>> Josiah Zayner
>>
>> http://DoItOurselfScience.blogspot.com
>>
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--
Cory Tobin
Graduate Student
Caltech :: Biology
ctobin@caltech.edu
805-443-4702
1200 E. California Blvd MC156-29
Pasadena CA, 91125

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