Re: [DIYbio] DIY nanodrop alternative?

It was the only peice of equipment I had to splurge on and buy new. Knowing how much DNA, RNA, Protein I have is paramount to almost everything in molecular biology. Too much has gone down the road of unpredictability because we use OD readings as absolute without a standard curve or using the OD 260/280 ratios for quantification. Its just not good enough for reliable results. Each spec is different so the readings on one can swing wildly on the other. I made the decision to use a system that does not care about salt noise and is, as advertised, 10k times more accurate than a nanodrop. Sure you are tied to the reagents but upon some investigation I found out that the dsDNA stain is a blue light induced safe stain. Im going to run some tests to see if you can use gelGreen or GelRed as the stain and TE buffer, which I think the nanodrop is using, as the assay reagents. The hardest part is making a DNA standard from scratch. Anyone savy enough may be able to hack the Qubit 2.0's firmware and make some raw data outputs and other tweaks. Bottom line is: IT IS WORTH ITS WEIGHT IN GOLD. That 2k hole burned a hole in my bank account for some time but it was well worth it. 

On Sun, Jul 14, 2013 at 10:31 PM, Dakota Hamill <dkotes@gmail.com> wrote:

The Qbit looks pretty cool.  Now that you linked it, I noticed it on the bench of another person in an abutting lab so I might have to ask him about it.  It definitely isn't cheap, but it also isn't the $5k-$15k a genquant or nanodrop look like they would cost.  $2k for a Qbit and 500 assays seems somewhat attainable if you don't have too many monthly bills.

Did you buy yours new Sebastian?

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