Re: [DIYbio] Meat Speciation for Newbies

Authentication of species in meat products by genetic techniques
Fátima C. Lago
European Food Research and Technology
March 2011, Volume 232, Issue 3, pp 509-515
http://link.springer.com/article/10.1007%2Fs00217-010-1417-1?LI=true#page-1
http://libgen.org/scimag2/10.1007/s00217-010-1417-1.pdf

Identification of Meat Species by Polymerase Chain Reaction (PCR) Technique
Turk. J. Vet. Anim. Sci.
2007; 31(3): 159-163
TÜBITAK
O. Irfan ILHAK**, Ali ARSLAN
http://journals.tubitak.gov.tr/veterinary/issues/vet-07-31-3/vet-31-3-3-0601-30.pdf

Detection of Adulteration and Identification of Cat's, Dog's, Donkey's
and Horse's Meat Using Species-Specific PCR and PCR-RFLP Techniques
Australian Journal of Basic and Applied Sciences, 3(3): 1716-1719, 2009
Salah M. Abdel-Rahman, Mohamed A. El-Saadani Khallid M. Ashry and
Amany S. Haggag
http://www.ajbasweb.com/ajbas/2009/1716-1719.pdf

On Mon, Jul 15, 2013 at 10:33 AM, Ewan <somebloke789@gmail.com> wrote:
> Hi All,
>
> A few people and I would like to do some meat speciation tests, primarily as
> a learning project for PCR. We don't know anything about primer design at
> all, though, and would appreciate any pointers at all to help us get
> started. We've got the machine, reagents, and electrophoresis figured out,
> but this looks like the hardest part right now. How do we get from here to a
> primer which will amplify sequence specific to, for instance, cows?
>
> I fully expect we'll need to do quite a bit of reading and messing around,
> which is fine, but we just don't know where to start.
>
> Thanks for any help,
>
> Ewan
>
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--
-Nathan

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