Re: [DIYbio] Radio-isotope and bacteria

Hmm, very neat idea. There is plenty of data on conjugating Rhenium to
proteins, so you might look into the virion surface proteins and
google scholar search that protein name with rhenium.

keywords: labelling radio-labelling radio conjugate phage virion

here are some papers I came up with just now

Labeling Biomolecules with Radiorhenium - a Review of the Bifunctional Chelators
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1949414/

http://diyhpl.us/~nmz787/pdf/A_Novel_Fluorescent_Probe_Europium_Complex_Hybridized_T7_Phage.pdf

http://en.wikipedia.org/wiki/Radioisotope_renography

http://www.freepatentsonline.com/y2013/0116408.html

http://f3.tiera.ru/2/B_Biology/BH_Human/Reilly%20R.M.%20(ed.)%20Monoclonal%20Antibody%20and%20Peptide-Targeted%20Radiotherapy%20of%20Cancer%20(Wiley,%202010)(ISBN%200470243724)(O)(659s)_BH_.pdf#page=128

http://www.google.com/patents?hl=en&lr=&vid=USPAT6706252&id=OQwRAAAAEBAJ&oi=fnd&dq=labelling+Rhenium+virus+envelope&printsec=abstract#v=onepage&q&f=false



I've used the protein linking kit at the end of this page before (with
plastic microspheres), it was quite simple:
http://www.bangslabs.com/products/accessory_reagents

That's assuming there are some free COOHs to bond with, they also sell
other kits for different chemistry. My point is, the linking chemistry
need not be too difficult in practice. Finding someone to try a
treatment that is novel/extraordinary is another thing.

Best luck, what you want is certainly possible, but you're gonna need
to find some specialist doctor who trusts molecular biologists and
maybe a chemist. Most MDs wouldn't know the carboxy terminus from the
amino of a protein... finding one that knows that much might be a
start.


On Thu, Jul 4, 2013 at 12:02 AM, Busuttil <kevin.j.busuttil@gmail.com> wrote:
> Hi Matthew,
>
> The main purpose is to use a radio-isotope, more specifically Rhenium-188,
> attached to a chicken pox strain to treat my wife for pancreatic cancer. My
> wife, 32 year old, was diagnosed with metastatic pancreatic cancer after
> giving birth to our only child. How difficult is it to find a lab that
> supplies an attentuated chicken pox virus with Rhenium-188?
>
> Thanks
>
> Kevin
>
> On Wednesday, 3 July 2013 15:25:10 UTC+1, Matthew Pocock wrote:
>>
>> Hi Kevin,
>>
>> The easiest way to radio label bacteria is to feed them on a radio labeled
>> carbon source. They will take it up and incorporate the isotope into its
>> body.
>>
>> Working with radioactivity would not be my first choice. You have to be
>> very careful how you dispose of everything and wear extra protection and
>> other safety precautions. Can I ask what you are wishing to do? There may be
>> a way to do it without radio labeling.
>>
>> Matthew
>>
>> Sent from my android - may contain predictive text entertainment.
>>
>> On 3 Jul 2013 15:11, "Busuttil" <kevin.j....@gmail.com> wrote:
>>>
>>> Hello everyone,
>>>
>>> What are the processes involved to attach a radio-isotope to bacteria?
>>>
>>> Kevin
>>>
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--
-Nathan

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