Re: [DIYbio] Re: PCR Question

Hi,

reviving this thread for one more question ;)


I did a PCR of a 9-10 kbp plasmid and the product should have been some 6300 bp long.

Now on this gel you see two bands (+primers), one at 9kbp and one at 6 kbp. May this be template plasmid + product? Or is this template (circular plasmid) + template (supercoiled) ?

Best,
Andreas

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---

You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/4KITIHKGNUk/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.

To post to this group, send email to diybio@googlegroups.com.

Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/4fb6d206-bb3c-4467-90a5-f4a77c6ad2d6%40googlegroups.com.

For more options, visit https://groups.google.com/groups/opt_out.