Re: [DIYbio] Re: PCR Question

If u want to avoid the 9kb band, try to use less PCR template as possible.

On Saturday, 13 July 2013 09:04:52 UTC+2, Mega wrote:

Here some pics...

On Sat, Jul 13, 2013 at 8:56 AM, Mega <masters...@gmail.com> wrote:

Hi,

reviving this thread for one more question ;)


I did a PCR of a 9-10 kbp plasmid and the product should have been some 6300 bp long.

Now on this gel you see two bands (+primers), one at 9kbp and one at 6 kbp. May this be template plasmid + product? Or is this template (circular plasmid) + template (supercoiled) ?

Best,
Andreas

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