Re: [DIYbio] Re: PCR Question

On Sunday, July 14, 2013 5:59:51 PM, shreyans chordia wrote:
> If u want to avoid the 9kb band, try to use less PCR template as possible.
>

I was going to say that too. Usually, you never see the plasmid
template band after pcr. What's your pcr enzyme and cycle conditions?
I'm suprised at the size. I would think it might have been better to
create multiple fragments and pcr combine them. Most likely will be
less prone to errors.

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