Re: [DIYbio] Re: restriction digest confirmation

Hmmm... I'm not sure you can tell from this gel.  It's possible that the plasmid DNA is nicked but not cut, so it's running at a higher apparent MW than if it were supercoiled.  

 

Can you extract the band in question from the gel?  Of course, you always lose some when you do that, but you may be able to make another cut to get the bits down to where you can see and size them.

 

 

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