Differentiated, but have you ever seen 2bp? The problem is not resolution, it is a mass problem. That the fragment is 6bp, and if you start out with 1 ug of dna that is 4kb long, then the weight of the 6bp fragment is 6/4000 ug, which is pretty small (1.5ng) and totally invisible. For reverence, i used 1 ug of dna for my digest, and used 1/5 of the digest for the gel, so we are down to .3 ng. Waaaayyyy below my ability to detect.
--AOn Tue, Jul 16, 2013 at 2:44 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Tue, Jul 16, 2013 at 11:36 AM, SC <stacy734@yahoo.com> wrote:I've seen as small as 2bp differentiated on agarose, but you need to
> Ah. My apologies, you did mention that in your orginal post, but my brain
> saw "6 kb" instead of "6 bp".
>
> I don't think you would ever be able to see such a small fragment on an
> agarose gel.
be careful about your reagents and use a really high % gel
http://diyhpl.us/~nmz787/pdf/Agarose-Based%20System%20for%20Separation%20of%20Short__97225pf01_10994a.pdf
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