Re: [DIYbio] Re: A simple and cheap >100V DIY electrophoresis power supply

Jonathan
I don't understand why you are continously trying to disparage me?
I did search online and there is not much info on using a voltage rectifier directly as a power source like I do in the circuit. I assume because it is pulsating DC and people would tend to not want to use pulsating DC?
The link Patrik posted(I think it was Patrik) has an almost identical circuit to mine except it has a single fuse and the website is meant for high school students it looks like? So I guess I am not the only one.

University of Chicago despite being one of the most prestigious schools in the world does not have any engineering departments.

There is no disregard for MSDS. Search online. Ethidium Bromide disposal is not regulated by the EPA. Most states do not have regulations regarding it's disposal either.

I am sure Ethidium Bromide is mutagenic if  inhaled in high quantities or consumed in large quantities or placed it on the skin in high quantities. I have worked with Ethidium Bromide for ~10 years and I can't recall it ever even touching my skin... I wear gloves as when working with it as I assume most people wear gloves when doing lab work especially with potentially hazardous chemicals? Again, it would seem only logical that if Ethidium Bromide was so dangerous there would be an outbreak of cancer amongst professional Scientists but there is not...

Anything else you want to attack me about?


On Thu, Jul 4, 2013 at 5:12 PM, Jonathan Cline <jcline@ieee.org> wrote:
One critical point about dealing with AC is that in a failure case, the circuit may not blow to create an open circuit.  There's a smaller probability esp with a bridge (~5% ?) that the circuit will fail as a short.  Thus creating a direct wire to the source of the AC.



On 7/4/13 2:59 PM, Simon Quellen Field wrote:

In my country, you won't get into legal trouble telling kids how much fun it is to French kiss light sockets. But that doesn't make it a good idea.
 
Well said.   Also it's disappointing to see this from a univ student where just walking over to another building to talk to some EE power systems guys and get the scoop (and an earful) first hand.   There are many older published articles on exactly the circuit suggested with similar caveats to everything said here tho obviously no homework was done, plus disregard for MSDS, that's what I think is the real bummer.   diybio is supposed to be self policing to avoid possible future government regulation and I think we just got told "shove it I'm gonna do what I want".



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