As part of some ongoing nerve conduction velocity experiments, I have been dissecting the nerves out of earthworms and trying to stain the fibers to get accurate measurements of the nerve diameter sizes. I have tried Golgi stains and cresyl violet, but the dyes are not penetrating the whole tissue very well. I think I may have to mount the nerve in an embedding block and then make small slices.
Making a DIY microtome is fairly simple, but I need to embed the tissue first to prepare for slicing. Any ideas for embedding recipes?
Tim
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