Hi everyone,
I was just thinking the following:
Salpetric acid causes mutations (it converts T -> U, then it's replicated error-pronely)
http://de.wikipedia.org/wiki/Pyrimidine#Abbau_und_Modifizierung_der_Basen
So if I keep the E. Coli with the glowing plasmid on LB-Amp-agar containing HNO2, they will mutate. At some time, ther may occur a transformant that glows yellow or purple or red or pink. That would be nice. Of course, for each "positive" transformant there will be hundreds that will have metabolic knock-outs and will die. And many which switch off the light (some fatal mutation in the luciferase)
Does that sound feasible?
How much HNO2 would you add? to pH 5.4? Or just very low?
-- I was just thinking the following:
Salpetric acid causes mutations (it converts T -> U, then it's replicated error-pronely)
http://de.wikipedia.org/wiki/Pyrimidine#Abbau_und_Modifizierung_der_Basen
So if I keep the E. Coli with the glowing plasmid on LB-Amp-agar containing HNO2, they will mutate. At some time, ther may occur a transformant that glows yellow or purple or red or pink. That would be nice. Of course, for each "positive" transformant there will be hundreds that will have metabolic knock-outs and will die. And many which switch off the light (some fatal mutation in the luciferase)
Does that sound feasible?
How much HNO2 would you add? to pH 5.4? Or just very low?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/ee5de01f-ea03-4726-bf2f-eb3d3baa80f8%40googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment