Thanks for the responses. I'm inclined to agree with Cathal here, the subject was "at home" tests - this necessarily negates anything that would require sending samples to an outside lab.
-- On another note, aren't there issues with saliva-based tests? It might just be marketing hype from some of the companies doing telomere-length testing, but I read from a few different places online that saliva-based tests were less accurate because they could only handle a more narrow selection of cells than blood-based tests and not all cells types age at the same rate.
On Friday, August 16, 2013 4:06:13 PM UTC-4, jlund256 wrote:
On Friday, August 16, 2013 4:06:13 PM UTC-4, jlund256 wrote:
There are several assays used for telomere length determination. Originally, DNA would be cut with a restriction enzyme that cuts in from the telomere repeat (in the subtelomeric region, I think), run on an agarose gel, and then Southern blotted and probed with telomere repeat sequence or a subtelomeric probe.Then telomere PCR was developed which would generate a ladder of bands, one for each repeat. Variants on this and qPCR based methods have also been developed.A method using ordinary PCR would be the easiest to implement as a DIY project.Here is a review:And more reviews: http://www.ncbi.nlm.nih.gov/pubmed?cmd=link& linkname=pubmed_pubmed_ reviews&uid=21663926&log$= relatedreviews&logdbfrom=pmc Jim Lund
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