Hi all,
I am designing primers to get a kanamycin resistance gene.
My question is, the kanamycin gene (nptII) is out of pGreenII for plants. I want it to work in E.Coli. Will the this eukaryotic RBS also work in E. Coli?
TGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAACTCGATCGAGGCATGATTGAACAA
As I need a BamHI site anyway, I could also mutagenize it into this...
TGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAACagGATCcAGGCATGATTGAACAA
The agga-motive rather looks like prokaryotic beings will accept it?
Or just leave it as it was?
-- I am designing primers to get a kanamycin resistance gene.
My question is, the kanamycin gene (nptII) is out of pGreenII for plants. I want it to work in E.Coli. Will the this eukaryotic RBS also work in E. Coli?
TGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAACTCGATCGAGGCATGATTGAACAA
As I need a BamHI site anyway, I could also mutagenize it into this...
TGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAACagGATCcAGGCATGATTGAACAA
The agga-motive rather looks like prokaryotic beings will accept it?
Or just leave it as it was?
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