That is true, but a) you could still use acetamide selection
b) attach a signal peptide to the host killing protein so it is secreted in the medium. If other cells take it up (can bacteria take up peptides, or just amino acids?) they die (except they have the surpression of killing protein from the plasmid)
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Re: [DIYbio] DIY and open Chymosin (Cheese Hacking) - anyone has some more experience
10:56 AM |
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